Fetal Fibronectin Specimen Collection

Fetal Fibronectin Specimen Collection1

Always collect the fetal fibronectin specimen before performing any other vaginal exam. Use of lubricating gels will interfere with the test results. 

Fetal fibronectin specimen collection kits are the only acceptable kits for specimen collection.

Order free Specimen Collection Kits

Specimen Collection Procedure

Step 1 Collect specimen prior to digital examination or manipulation of the cervix to avoid sample contamination.

Step 2 During speculum exam, lightly rotate swab across posterior fornix of the vagina for 10 seconds to absorb cervicovaginal secretions.

Step 3 Remove swab and immerse tip in buffer. Break the shaft at the score even with the top of the tube.

Step 4 Insert the swab shaft into the hole inside the tube cap and push down tightly over the shaft, sealing the tube with a click. Ensure the shaft is inserted securely to avoid leakage. Label, and send fetal fibronectin sample to a lab near you.

For Accurate Patient Results

For accurate patient results, please ensure that you follow these specimen collection instructions.

  • The specimen should be collected prior to a digital cervical exam, collection of culture specimens, or vaginal probe ultrasound exams. 
  • Do not contaminate the swab or specimen with lubricants, soaps, disinfectants, or creams.
  • Do not collect specimen if patient has had sexual intercourse within 24 hours prior to sampling*; moderate or gross vaginal bleeding; advanced cervical dilation (3 cm or greater); rupture of membranes; gestational age before 22 weeks or after 35 weeks; or suspected or known placental abruption or placenta previa.
  • *If the test result is negative, it is a valid result.

For a complete description of the Specimen Collection Procedure and related Precautions and Warnings and Contraindications relating to patient eligibility, please refer to the Specimen Collection Kit Insert and the Rapid fFN Cassette Directional Insert.


  1. The Hologic Specimen Collection Kit, instructions for use. PI AW-04196-003. Marlborough, MA; Hologic, Inc., 2015.

Preterm Birth Webinars

Leading clinicians discuss timely topics on the management of preterm birth.

View on hologiceducation.com


This link will take you to the information intended for healthcare professionals outside of the United States. Because these pages concern the international distribution activities of the QuikCheckTM fFN Test, which are not necessarily governed by US law, contents of the international pages may contain information that does not pertain to US users, including certain products that may not be cleared or approved by US Food & Drug Administration for promotion or sale in the United States. Click “Continue” to proceed, or click “Cancel” to remain in the US site.

Caution: The QuikCheck fFNTest is not sold or offered for sale in the United States or Canada.



You Are Leaving a Hologic Website

Hologic, Inc. does not review nor control the content on non-Hologic websites. This link does not constitute an endorsement by Hologic of the content of the site you are about to visit. Hologic privacy procedures do not apply to the owners of a non-Hologic website.